Journal: Microbiome

Article Title: Ochratoxin A induces liver inflammation: involvement of intestinal microbiota

doi: 10.1186/s40168-019-0761-z

Figure Lengend Snippet: OTA promotes liver inflammation. a Relative mRNA expressions of TLR4, MYD88, IKBα, IL-6, and TNF-α in the liver after OTA oral gavage ( n = 6, mean with SEM). b Relative protein abundances of TLR4, MYD88, p-IKBα, p-IKBα/IKBα, and p-p65 in the liver after OTA oral gavage ( n = 6, mean with SEM). c Effect of OTA on levels of liver cytokines, including IL-1β, IL-6, TNF-α, IL-8, IL-10, and IFN-γ ( n = 6, mean with SEM). d Representative images of H&E-stained liver sections in CON and OTA group (magnification × 400, scale bar 100 μm, n = 6). e Statistical analysis of the percentage of inflammatory cells in different groups shown in d ( n = 6, mean with SEM). f Effect of OTA on serum levels of AST, ALT, ALP, and LDH ( n = 6, mean with SEM). g Serum LPS level with or without OTA treatment ( n = 6, mean with SEM). h Effect of OTA on serum levels of IL-1β, IL-6, TNF-α, and IL-10 ( n = 6, mean with SEM). Data in a , b , c , e , f , g , and h were analyzed with unpaired t test, * P < 0.05; ** P < 0.01, *** P < 0.001, P <0.0001

Article Snippet: Proteins were loaded onto the SDS-PAGE gel (BioRad) and electrophoresed and analyzed by WB using antibodies against TLR4 (BA1717, Boster, Wuhan, China), MYD88 (abs135682, Absin, Shanghai, China), IKBα (D120138, Sangon Biotech, Shanghai, China), p-IKBα (D151548, Sangon Biotech, Shanghai, China), p-p65 (HZ4902812, TW reagent, Shanghai, China), TJP-1 (mAb13663, Cell Signaling, USA), Occludin (ab167161, Abcam, USA), and actin (60008, Proteintech, USA).

Techniques: Staining

Journal: Microbiome

Article Title: Ochratoxin A induces liver inflammation: involvement of intestinal microbiota

doi: 10.1186/s40168-019-0761-z

Figure Lengend Snippet: OTA fails to promote liver inflammation in antibiotics-treated ducks. a Liver LPS level in different groups ( n = 6, mean with SEM). b Relative mRNA expressions of TLR4, MYD88, IKBα, IL-6, and TNF-α in the liver of antibiotics-treated ducks with or without OTA ( n = 6, mean with SEM). c Levels of IL-1β, IL-6, TNF-α, IL-8, IL-10, and IFN-γ in the liver of antibiotics-treated ducks with or without OTA ( n = 6, mean with SEM). d Representative images of H&E-stained liver sections (magnification × 400; scale bar 100 μm; n = 6). e Statistical analysis of the percentage of inflammatory cells in different groups shown in d ( n = 6, mean with SEM). f Effect of OTA on serum levels of AST, ALT, ALP and LDH in antibiotics-treated ducks ( n = 6, mean with SEM). g Serum LPS level with or without OTA treatment in antibiotics-treated ducks ( n = 6, mean with SEM). h Effect of OTA on serum levels of IL-1β, IL-6, TNF-α, and IL-10 ( n = 6, mean with SEM). Data were analyzed with unpaired t test. n.s., not significant

Article Snippet: Proteins were loaded onto the SDS-PAGE gel (BioRad) and electrophoresed and analyzed by WB using antibodies against TLR4 (BA1717, Boster, Wuhan, China), MYD88 (abs135682, Absin, Shanghai, China), IKBα (D120138, Sangon Biotech, Shanghai, China), p-IKBα (D151548, Sangon Biotech, Shanghai, China), p-p65 (HZ4902812, TW reagent, Shanghai, China), TJP-1 (mAb13663, Cell Signaling, USA), Occludin (ab167161, Abcam, USA), and actin (60008, Proteintech, USA).

Techniques: Staining

Journal: Microbiome

Article Title: Ochratoxin A induces liver inflammation: involvement of intestinal microbiota

doi: 10.1186/s40168-019-0761-z

Figure Lengend Snippet: OTA-originated microbiota induces liver inflammation. a Liver LPS level in FMT (CON) and FMT (OTA) ducks. b Relative mRNA expressions of TLR4, MYD88, IKBα, IL-6, and TNF-α in the liver after FMT ( n = 6, mean with SEM). c Relative protein abundance of TLR4, MYD88, p-IKBα, p-IKBα/IKBα, and p-p65 in the liver after FMT ( n = 6, mean with SEM). d Effect of FMT on the liver levels of IL-1β, IL-6, TNF-α, and IL-10 ( n = 6, mean with SEM). e Representative H&E-stained liver sections. f Statistical analysis of the percentage of inflammatory cells in different groups shown in e ( n = 6, mean with SEM). g Serum levels of AST, ALT, ALP, and LDH in different groups ( n = 6, mean with SEM). h Serum LPS level in different FMT groups ( n = 6, mean with SEM). i Serum levels of IL-1β, IL-6, and TNF-α after FMT ( n = 6, mean with SEM). FMT (CON): ducks received the CON group fecal microbiota. FMT (OTA): ducks received the OTA group fecal microbiota. Data were analyzed with unpaired t test, n = 6. * P < 0.05; ** P < 0.01, *** P < 0.001, **** P <0.0001

Article Snippet: Proteins were loaded onto the SDS-PAGE gel (BioRad) and electrophoresed and analyzed by WB using antibodies against TLR4 (BA1717, Boster, Wuhan, China), MYD88 (abs135682, Absin, Shanghai, China), IKBα (D120138, Sangon Biotech, Shanghai, China), p-IKBα (D151548, Sangon Biotech, Shanghai, China), p-p65 (HZ4902812, TW reagent, Shanghai, China), TJP-1 (mAb13663, Cell Signaling, USA), Occludin (ab167161, Abcam, USA), and actin (60008, Proteintech, USA).

Techniques: Quantitative Proteomics, Staining

Journal: Aging (Albany NY)

Article Title: LCZ696 ameliorates lipopolysaccharide-induced endothelial injury

doi: 10.18632/aging.202692

Figure Lengend Snippet: Cells were stimulated with LPS (1 μg/mL) in the presence or absence of LCZ696 (20 μM) for 24 hours. ( A ). The levels of TLR4 and Myd88 were measured using Western blot; ( B ). Nuclear levels of NF-κB p65 ( *** P < 0.005 vs. control group; ##, ### P < 0.01, 0.005 vs. LPS group).

Article Snippet: The primary antibodies include: TLR4 (1:1000, #14358, Cell Signaling Technologies, USA), Myd88 (1:2000, #50010, Cell Signaling Technologies, USA), NF-κB p65 (1:2000, #8242, Cell Signaling Technologies, USA), Lamin B1 (1:3000, #MAB8525, R&D systems, USA) and β-actin (1:10000, #MAB8929, R&D systems, USA).

Techniques: Western Blot, Control